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Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem
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Chapters
- 00:05Title
- 01:53Preparations Prior to Infiltration
- 03:40Syringe Infiltration
- 05:57Sampling Leaf Tissue and Quantifying Pathogen Growth
- 09:11Results: Syringe Infiltration Assays can Quantitatively Assess Plant Immune Response
- 10:33Conclusion
Quantification of pathogen growth is a powerful tool to characterize various Arabidopsis thaliana (hereafter: Arabidopsis) immune responses. The method described here presents an optimized syringe infiltration assay to quantify the Pseudomonas syringae pv. maculicola ES4326 growth in adult Arabidopsis leaves.
