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Measuring Phagosome pH by Ratiometric Fluorescence Microscopy
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Measuring Phagosome pH by Ratiometric Fluorescence Microscopy

Measuring Phagosome pH by Ratiometric Fluorescence Microscopy

DOI:
10.3791/53402-v

•

14:39 min

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December 07, 2015

•

, ,
1Department of Cellular Physiology and Metabolism,University of Geneva

Chapters

  • 00:05Title
  • 01:02Preparation of Phagocytic Targets
  • 04:18Live Video Microscopy
  • 05:47Calibration and Control Experiments
  • 06:34Analysis
  • 11:44Results: Time Courses, Average pH and Ratio Histograms of Wild-type and Hvcn1-/- Neutrophil Phagosomes
  • 13:08Conclusion

Summary

자동 번역

자동 번역

Phagosomal pH influences phagosome maturation, oxidant production, phagosomal killing as well as antigen presentation. Here we describe a ratiometric method for measuring time-course and endpoint pH changes in individual phagosomes in living phagocytes using fluorescence microscopy.

Tags

PhagosomePHRatiometric Fluorescence MicroscopyPhagocytosisNeutrophilsFITCZymosanPH MeasurementLive-cell ImagingIntracellular PathogensPhagosomal AcidificationFluorescent Probes

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