JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 00:48Protocol for Interphasic Xenopus Laevis Egg Extract
- 05:38Protocol for In Vitro Reconstitution of Chromatin Decondensation
- 08:56Results: Chromatin Decondensation is ATP and GTP Dependent
- 09:48Conclusion
The molecular mechanisms of the decondensation of highly compacted mitotic chromatin are ill-defined. We present a cell-free assay based on mitotic chromatin clusters isolated from HeLa cells and Xenopus laevis egg extract that faithfully reconstitutes the decondensation process in vitro.
