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Chapters
- 00:05Title
- 00:36Brain Sample Homogenization and Differential Ultracentrifugation
- 04:37Immunoblotting of Samples
- 07:25Results: Distinct Species of Alpha-Synuclein Detected by Western Immunoblot
- 08:35Conclusion
Here, we present a protocol for the isolation of increasingly insoluble/aggregated alpha-synuclein (α-syn) from post-mortem human brain tissue. Through the utilization of buffers with increasing detergent strength and high-speed ultracentrifugation techniques, the variable properties of α-syn aggregation in diseased and non-diseased tissue can be examined.
