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Chapters
- 00:05Title
- 01:01Sodium Carbonate Extraction of Intact Mitochondria
- 03:07Separation of Inner and Outer Membranes of Mitochondria
- 04:22Construction of Mitochondria-targeted GFP/RFP-tagged CyclinB1/Cdk1 Vectors and Mitochondria Localization
- 07:23Determination of Cell Cycle Phase Lengths with EdU Incorporation Assay
- 10:58Results: Mitochondrial Analysis of CyclinB1 and Cdk1
- 12:25Conclusion
Here, we outline how to study mitochondrial localization of a (cell cycle) kinase, and how to determine its sub-mitochondrial location as well as potential mitochondrial substrates/targets. Forced expression of proteins into the mitochondria provides a useful tool for studying the functional consequences of mitochondrial localization of a protein of interest.
