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鸡小脑切片的体外培养和空间上有针对性的颗粒细胞前体电穿孔

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Ex Vivo Culture of Chick Cerebellar Slices and Spatially Targeted Electroporation of Granule Cell Precursors

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鸡小脑切片的体外培养和空间上有针对性的颗粒细胞前体电穿孔

DOI:

10.3791/53421-v

•

10:02 min

•

December 14, 2015

•

Michalina Hanzel, Richard J.T. Wingate, Thomas Butts

¹MRC Centre of Developmental Neurobiology,King’s College London, ²School of Biological and Chemical Sciences,Queen Mary, University of London

Chapters

00:05Title
00:55Dissection of E14 Cerebellum
03:00Slice Culture of E14 Cerebellum
04:21Electroporation of Cerebellum Slices
07:08Preparing Cerebellar Slices for Imaging
07:58Results: Cerebellar Tissue Maintains Integrity and Proliferation in Culture
09:14Conclusion

Summary

자동 번역

小脑外部颗粒层是最大的过境扩增在显影脑的部位。在这里，我们提出了一个协议，以目标基因改造这个层扩散采用体外电击和小脑切片文化从胚胎第14天鸡胚的高峰。

Tags

Cerebellar Slices Granule Cell Precursors Ex Vivo Culture Electroporation Cerebellar External Granule Layer Neuronal Proliferation Differentiation Evo-devo Medulloblastoma Granule Neuron Development Cerebellum Development Evolution Disease

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