Journal
/
/
Endotoksin ve DNA İçeriği için Hava örneklenmiş Filtresi Analizi
JoVE 신문
환경과학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 환경과학
Air-sampled Filter Analysis for Endotoxins and DNA Content
DOI:

09:16 min

March 07, 2016

, , ,

Chapters

  • 00:05Title
  • 00:34Air Filter Preparation and Sampling
  • 01:38Extraction of Endotoxin from Filter Samples
  • 02:51Limulus Amebocyte Lysate Endotoxin Test
  • 04:30DNA Extraction from Experimental and Spiked Filters
  • 06:02Quantitative PCR Analysis
  • 07:48Results: Centrifugation Speed and Filter Load Influences the Measured Endotoxin Concentrations in Samples
  • 08:35Conclusion

Summary

자동 번역

Two complementary analyses of atmospheric biological particles from air sampled filters are described herein: the extraction and detection of endotoxin, and of DNA.

Related Videos

Read Article