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Transformation of Probiotic Yeast and Their Recovery from Gastrointestinal Immune Tissues Following Oral Gavage in Mice
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Transformation of Probiotic Yeast and Their Recovery from Gastrointestinal Immune Tissues Following Oral Gavage in Mice
DOI:

12:12 min

February 08, 2016

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Chapters

  • 00:05Title
  • 01:00Generate Survival Curves to Determine Needed Doses of UV Irradiation
  • 03:01Screening for Mutagenized Auxotrophic Yeast Strains and Yeast Transformation
  • 05:42Oral Gavage of Mice with Transformed Yeast
  • 07:50Harvest of Murine Peyer’s Patches and Isolation of Viable Yeast Colonies
  • 10:03Representative Results
  • 11:28Conclusion

Summary

자동 번역

Presented here is a unified description of techniques that can be used to develop, transform, administer, and test heterologous protein expression of the probiotic yeast Saccharomyces boulardii.

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