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Chapters
- 00:05Title
- 01:14Xenopus laevis Tadpole Anterior Neutral Tube Flat-mounting
- 03:05Animal Cap Explant Induction Using Anion Exchange Resin Beads
- 04:58Animal Cap Dissociation and Reaggregation
- 06:52Animal Cap Explant Implantation onto Xenopus laevis Embryo Neural Plates
- 08:12Results: Representative Xenopus Explants
- 10:13Conclusion
In Xenopus embryos, cells from the roof of the blastocoel are pluripotent and can be programmed to generate various tissues. Here, we describe protocols to use amphibian blastocoel roof explants as an assay system to investigate key in vivo and in vitro features of early neural development.
