Journal
/
/
Beeldverwerking en flowcytometrie-gebaseerde analyse van de Cel standpunt en de celcyclus in 3D Melanoom Sferoïden
JoVE 신문
의학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 의학
Imaging- and Flow Cytometry-based Analysis of Cell Position and the Cell Cycle in 3D Melanoma Spheroids
DOI:

10:44 min

December 28, 2015

, , , ,

Chapters

  • 00:05Title
  • 01:063D Spheroid Formation
  • 03:30Image Analysis of FUCCI Spheroid Sections
  • 08:40Results: Quantification of G1 Arrested and Proliferating Cells in Cancer Cell Spheroids
  • 10:00Conclusion

Summary

자동 번역

We describe two complementary methods using the fluorescence ubiquitination cell cycle indicator (FUCCI) and image analysis or flow cytometry to identify and isolate cells in the inner G1 arrested and outer proliferating regions of 3D spheroids.

Related Videos

Read Article