Journal
/
면역학 및 감염병학
/
Isolering av Exosomes fra Plasma av HIV-1 Positive Personer
JoVE 신문
면역학 및 감염병학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 면역학 및 감염병학
Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals

Isolering av Exosomes fra Plasma av HIV-1 Positive Personer

DOI:
10.3791/53495-v

•

06:46 min

•

January 05, 2016

•

, , , , , ,
1Department of Microbiology, Biochemistry, Immunology,Morehouse School of Medicine, 2Department of Medicine,Emory University School of Medicine, 3Department of Pathology and Laboratory Medicine,Emory University School of Medicine, 4Yerkes National Primate Research Center

Chapters

  • 00:05Title
  • 00:54Human Blood Processing
  • 02:20Purification of Exosomes
  • 03:41Acetylcholinesterase Activity Assay
  • 04:50Results: Characterization of Exosomes from HIV-1 Positive Human Plasma
  • 06:10Conclusion

Summary

자동 번역

자동 번역

Techniques describing a gradient procedure to separate exosomes from human immunodeficiency virus (HIV) particles are described. This procedure was used to isolate exosomes away from HIV particles in human plasma from HIV-infected individuals. The isolated exosomes were analyzed for cytokine/chemokine content.

Tags

Keywords: ExosomesHIV-1PlasmaVirus ParticlesUltracentrifugationIodixanol GradientVirus-infected CellsMicrovesiclesHIV InfectionCentrifugationPBS WashVirus Separation

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/53495?article_type=v

Assessment of Immunologically Relevant Dynamic Tertiary Structural Features of the HIV-1 V3 Loop Crown R2 Sequence by ab initio Folding
check_url/kr/53495?article_type=v

Specific Marking of HIV-1 Positive Cells using a Rev-dependent Lentiviral Vector Expressing the Green Fluorescent Protein
check_url/kr/53495?article_type=v

Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3
check_url/kr/53495?article_type=v

Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays
check_url/kr/53495?article_type=v

Prediction of HIV-1 Coreceptor Usage (Tropism) by Sequence Analysis using a Genotypic Approach
check_url/kr/53495?article_type=v

In vitro Uncoating av HIV-1 Cores
check_url/kr/53495?article_type=v

Nye verktøy for å utvide Regulatoriske T-celler fra HIV-1-infiserte individer
check_url/kr/53495?article_type=v

Conformational Evaluering av HIV-1 Trimeric kappeglykoproteiner Ved hjelp av en Cell-basert ELISA-analysen
check_url/kr/53495?article_type=v

Nucleocapsid annealing-mediert elektroforese (NAVN) analysen Lar Rapid Identifikasjon av HIV-1 nucleocapsid hemmere
check_url/kr/53495?article_type=v

Visualisering av HIV-1 Gag Binding til Giant unilamelære vesikkel (GUV) Membraner

Read Article