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एक साथ दो-फोटान<em> Vivo</em> माउस Retrosplenial कोर्टेक्स में अन्तर्ग्रथनी आदानों की इमेजिंग और postsynaptic लक्ष्य
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신경과학
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JoVE 신문 신경과학
Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex
DOI:

16:45 min

March 13, 2016

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Chapters

  • 00:00Title
  • 01:27Surgery Preparation
  • 03:37Cranial Window Surgery
  • 08:30Virus Injection
  • 10:43Cranial Window Implantation
  • 13:15Imaging
  • 15:12Results
  • 15:56Conclusions

Summary

자동 번역

This video shows the craniotomy procedure that allows chronic imaging of neurons in mouse retrosplenial cortex using in vivo two photon microscopy in Thy1-GFP transgenic line. This approach is combined with injection of mCherry-expressing adeno-associated virus into dorsal hippocampus. These techniques allow long-term monitoring of experience-dependent structural plasticity in RSC.

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