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Chapters
- 00:05Title
- 01:00Primary Human Mesothelial Cell (HPMC) Isolation and Culture
- 03:57Normal Omentum Fibroblast (NOF) Isolation and Culture
- 04:54Organotypic Culture Plating
- 06:47Results: The Role of Integrins in Ovarian Cancer Cell Adherence, Proliferation and Invasion
- 07:59Conclusion
Here, we present a protocol to construct a three-dimensional in vitro model of the lining of the peritoneal cavity, composed of primary human mesothelial cells and fibroblasts layered with extracellular matrix, as a tool to investigate ovarian cancer cell adhesion, invasion, and proliferation.
