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حل الحيوي النووية الاستقطاب الأجهزة لفي الوقت الحقيقي القياسات الأنزيمية ردود الفعل قيم وNMR
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JoVE 신문 화학
Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
DOI:

10:54 min

February 23, 2016

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Chapters

  • 00:05Title
  • 01:37Polarization
  • 03:29Solid-state NMR Measurements
  • 05:15Sample Preparation and Positioning
  • 06:19Dissolution and Injection
  • 07:32Liquid-state NMR Measurement
  • 08:25Results: Real-time Enzymatic Reaction Rate Measurement for LDH Activity
  • 09:08Conclusion

Summary

자동 번역

The sensitivity enhancement provided by dissolution dynamic nuclear polarization (DNP) enables following metabolic processes in real time by NMR and MRI. The characteristics and performances of a dedicated dissolution DNP setup designed for study enzymatic reactions are discussed.

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