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Fast Enzymatisk Behandling av proteiner for MS Detection med en Gjennomstrømning mikroreaktor
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JoVE 신문 생체공학
Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor
DOI:

09:49 min

April 06, 2016

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Chapters

  • 00:05Title
  • 00:41Preparation of the Capillary Microreactor
  • 03:07Experimental Setup
  • 05:03Sample Loading, Proteolytic Digestion, and Elution for MS Analysis
  • 07:05Data Processing
  • 07:50Results: Mass Spectrometry Protein Detection Using a Flow-through Microreactor
  • 08:11Conclusion

Summary

자동 번역

A quick protocol for proteolytic digestion with an in-house built flow-through tryptic microreactor coupled to an electrospray ionization (ESI) mass spectrometer is presented. The fabrication of the microreactor, the experimental setup and the data acquisition process are described.

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