JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 00:52Culture Inoculation
- 03:00Cell Maintenance: Checking Cell Density and Viability and Passaging Cells
- 05:11Transfection
- 07:50Expression and Harvest
- 08:36Results: High Yield Expression of Appropriately Glycosylated Protein with Transient Transfection
- 10:20Conclusion
Laboratory-scale production of eukaryotic proteins with appropriate post-translational modification represents a significant barrier. Here is a robust protocol with rapid establishment and turnaround for protein expression using a mammalian expression system. This system supports selective amino acid, selective labeling of proteins and small molecule modulators of glycan composition.
