Journal
/
생물학
/
减数分裂纺锤体评估在小鼠卵母细胞的siRNA介导沉默
JoVE 신문
생물학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생물학
Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing
Please note that all translations are automatically generated. Click here for the English version.

减数分裂纺锤体评估在小鼠卵母细胞的siRNA介导沉默

DOI:
10.3791/53586-v

•

09:16 min

•

October 11, 2015

•

,
1Department of Physiology and Pharmacology,College of Veterinary Medicine, University of Georgia

Chapters

  • 00:05Title
  • 00:54Collecting Mouse Oocytes
  • 02:08Oocyte Microinjection
  • 04:42Culturing and Staining the Injected Oocytes
  • 07:52Results: Disruption of Pericentrin Expression in Oocytes during Meiotic Division
  • 08:37Conclusion

Summary

자동 번역

자동 번역

在这里，我们提出了一个协议，用于特定的siRNA介导的mRNA枯竭其次是免疫荧光分析来评估减数分裂纺锤体组装和组织在小鼠卵母细胞。这个协议是适合体外转录耗尽的和不同的主轴和/或微管组织中心相关因子中的卵母细胞的功能评价。

Tags

Meiotic SpindleSiRNAChromosome SegregationAneuploidyMicrotubule Spindle ApparatusMTOCMouse OocytesImmunofluorescence Analysis

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

小鼠卵母细胞的细胞核移植到

检索小鼠卵母细胞

收集和仓鼠卵母细胞和小鼠胚胎冷冻保存

活体成像和治疗卵巢癌的原位小鼠模型

膜片钳和灌注技术研究离子通道的表达爪蟾卵母细胞

流动小鼠减数分裂细胞的流式细胞仪净化

活细胞钙成像相结合的siRNA介导的基因沉默标识CA 2 +泄漏通道内质网膜和监管机制

农杆菌介导的病毒诱导的基因沉默含量在棉花

长期沉默的1S Intersectin的在小鼠肺的特异性siRNA，通过阳离子脂质体重复交货。通过电子显微镜的击倒效果评价

在造血干细胞和祖细胞的成年小鼠颅骨骨髓体内四维跟踪

Read Article