JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Departments of Chemistry and Molecular Biosciences, Chemistry of Life Processes Institute, Proteomics Center of Excellence, Robert H. Lurie Comprehensive Cancer Center,Northwestern University, 2Institute of Chemistry, Proteomics Unit,Federal University of Rio de Janeiro, 3Department of Cell Biology, Brazilian Center for Protein Research, Laboratory of Biochemistry and Protein Chemistry,University of Brasilia
Chapters
- 00:05Title
- 00:38Prepararing the Gradient Tube Gels
- 04:44Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis (CN-GELFrEE) Setup
- 06:59CN-GELFrEE: Sample Loading to Analysis
- 09:13Results: Non-covalent Protein Assemblies from Mouse Heart
- 11:00Conclusion
This protocol describes how to prepare and perform clear native gel-eluted liquid fraction entrapment electrophoresis (CN-GELFrEE), a native separation technique for non-covalent biomolecular assemblies and proteins from heterogeneous samples that is compatible with various downstream protein analysis techniques.
