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对小鼠淋巴瘤模型监测肿瘤进展和治疗效果生物发光基于肿瘤定量方法

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Bioluminescence-Based Tumor Quantification Method for Monitoring Tumor Progression and Treatment Effects in Mouse Lymphoma Models

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对小鼠淋巴瘤模型监测肿瘤进展和治疗效果生物发光基于肿瘤定量方法

DOI:

10.3791/53609-v

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07:12 min

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July 07, 2016

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Jeremie Cosette^2,3, Rym Ben Abdelwahed, Sabrina Donnou-Triffault^4,5, Catherine Sautès-Fridman, Patrice Flaud*³, Sylvain Fisson*^1,4,5

¹Genethon, ²Cordeliers Research Center,INSERM U872, ³Matter and Complex Systems Laboratory,Université Paris Diderot, ⁴INSERM U951, ⁵UMR_S951,Université d’Evry Val d’Essonne

Chapters

00:05Title
00:48Bioluminescence Imaging
02:40Bioluminescence Quantification and Image Analysis
03:59Results: Representative Tumor Burden Monitoring and Treatment Response in Various Mouse Lymphoma Models
06:10Conclusion

Summary

자동 번역

生物发光成像是用于定位肿瘤和转移一个众所周知的工具，但是这些图像的量化通常需要复杂的计算和特定仪器。我们描述了易于使用luminoscore方法，基于精确采集条件，不需要计算，并且使肿瘤负荷和处理响应于在小鼠模型中进行监测。

Tags

Bioluminescence-based Tumor Quantification Method Monitoring Tumor Progression Treatment Effects Mouse Lymphoma Models Bioluminescence Acquisition Procedures Image Analyses Quantitative Data Reproducible Quantification Of Imaging Data Advantages Of The Technique Easy Setup In Any Animal Facility Does Not Require Complex Calculations Imager Initialization Sedation Level Confirmation D-Luciferin Potassium Salt Solution Injection Firefly Luciferase Expression In Tumor Cells Light Production And Detection With The Imager Imaging Of Posterior And Anterior Body Positions

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