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Chapters
- 00:05Title
- 00:58Generation and Expression of Fusion Proteins and Free Dam Proteins
- 03:12Library Preparation: Fragmentation
- 05:29Library Preparation: Size Selection
- 07:26Results: Detecting Lamin B1 Binding Regions on the Mouse Chromosome One
- 08:20Conclusion
Vi beskriver häri en analys genom att koppla DNA adenin metyltransferas identifiering (DamID) till hög genomströmning sekvensering (DamID-punkter). Denna förbättrade metod ger en högre upplösning och ett bredare dynamiskt omfång, och låter analysera DamID-seq uppgifter i samband med andra high throughput sekvense data såsom Chip-punkter, RNA-punkter, etc.
Tags
DamID-seqGenome-wide MappingProtein-DNA InteractionsHigh Throughput SequencingAdenine-methylated DNA FragmentsNuclear OrganizationTranscription RegulationMammalian CellsGenomic RegionsAmyloidTranscription FactorsHigh ResolutionDNA MicroarraysCross-analysisGene CloningDamID VectorLentiviral StocksLentiviral Expression SystemCotransfectionLentivirus Infection
