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Chapters
- 00:05Title
- 01:19Preparation of Acute Tilted Amygdala Slices
- 03:33Preparation of Coronal Amygdala Slices
- 04:50Visualization and Stimulation of Presynaptic Fibers
- 08:04Results: Optogenetic Activation of Long Range and Local Projections
- 10:02Conclusion
Optogenetic approaches are widely used to manipulate neural activity and assess the consequences for brain function. Here, a technique is outlined that upon in vivo expression of the optical activator Channelrhodopsin, allows for ex vivo analysis of synaptic properties of specific long range and local neural connections in fear-related circuits.
Tags
Ex Vivo OptogeneticsFear CircuitsBrain SlicesNeural ProjectionsMicro-circuitsChannel RhodopsinPatch-clamp RecordingsSynaptic PropertiesLocal ConnectivityLong-range ConnectivityBrain AreasConventional Electrical Stimulation TechniquesViral InjectionsExpression TimeBrain Slices PreparationLight Activation And RecordingsRecombinant Adeno-associated Viral VectorMedial Pre-frontal CortexVibratome
