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Chapters
- 00:05Title
- 00:57Primary Cell Culture and Alginate Encapsulation
- 04:04Liberate Cells from Alginate and Actin Cytoskeleton Arrangement
- 05:54Results: Human Pituitary Adenoma Cells in Three-dimensional Aliginate Bead Culture
- 07:09Conclusion
Three-dimensional culture in alginate beads, due to its simplicity and reproducibility, was chosen to maintain the pituitary adenoma cells. The procedures included an initial enzymatic digestion and mechanical dissociation of the tumor tissue, and the subsequent cell suspension was encapsulated in alginate beads.
Tags
Three-dimensionalAlginate-bead CultureHuman Pituitary Adenoma CellsTumor Cell BiologyMarkers Of InvasivenessAlginate ScaffoldInvestigationCarmen Solano-AgamaResearch AssistantLaminar Flow CabinetCalcium-and Magnesium-free PBSSurgical TweezersPetri DishRed Blood CellsDebrisSurgical ScissorSmall PiecesPasteur PipetteTubeCentrifugeCollagenase Solution
