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Isolation der infiltrierenden Leukozyten aus Mäusehaut unter Verwendung enzymatischer Digest und Gradiententrennung
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 00:58Flank Skin Harvest and Infiltrating Leukocyte Isolation
- 04:28Results: Oxazolone (Ox) Challenge Results in Pronounced T Cell and Neutrophil Flank Skin Infiltration
- 06:17Conclusion
This protocol describes enzymatic digestion of mouse skin in nutrient-rich medium followed by gradient separation to isolate leukocytes. Cells thus derived can be used for diverse downstream applications. This is an effective, economical, and improved alternative to tissue dissociation machines and harsher trypsin and dispase-based tissue digestion protocols.
Tags
IsolationInfiltrating LeukocytesMouse SkinEnzymatic DigestGradient SeparationCellular MechanismsMolecular MechanismsMouse ModelsSkin PathologiesAtopic DermatitisResident Cell PopulationsSkin HarvestingVisual DemonstrationPrecisionPracticeSurgical ScissorsFlank SkinND4 Swiss-Webster MouseSurgical Blade50 Milliliter Conical TubeHBSSEDTAHEPESFBS Incubation
