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提高2D和3D皮肤<em>体外</em>模型使用大分子拥挤

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Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding

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提高2D和3D皮肤<em>体外</em>模型使用大分子拥挤

DOI:

10.3791/53642-v

•

09:14 min

•

August 22, 2016

•

Paula Benny², Cedric Badowski, E. Birgitte Lane, Michael Raghunath^2,3

¹Department of Biochemistry, Yong Loo Lin School of Medicine,National University of Singapore, ²Epithelial Biology Laboratory,Institute of Medical Biology, A*STAR, ³Department of Biomedical Engineering, Faculty of Engineering,National University of Singapore

Chapters

00:05Title
01:16Macromolecular Crowding in 2D Skin Cell Cultures
02:06Making and Using a Cell-derived Matrix
03:393D Organotypic Skin Co-culture
06:24Results: Macromolecular Crowding of Fibroblasts and Keratinocytes
08:01Conclusion

Summary

자동 번역

我们提出了一个协议，以获得富含胞外基质蛋白的细胞来源的基质，使用大分子沟渠扫污机（MMC）。此外，我们提出该结合MMC在三维器官皮肤共培养产生，从而降低了培养时间，同时保持结构的成熟的协议。

Tags

Macromolecular Crowding Extracellular Matrix Deposition 2D And 3D Skin Models Cell-derived Matrices Organotypic Skin Constructs Wound Therapies Skin Equivalents Primary Fibroblasts Primary Keratinocytes Co-culture Decellularization Sodium Deoxycholate Cell-derived Matrix

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