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Journal / Bioengineering / La mejora en 2D y 3D de la piel
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Bioengineering

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JoVE Journal Bioengineering
La mejora en 2D y 3D de la piel
 
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La mejora en 2D y 3D de la piel

Article DOI: 10.3791/53642-v • 09:14 min • August 22nd, 2016
August 22nd, 2016 •
1,2, 2, 2, 1,2,3
1Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 2Epithelial Biology Laboratory, Institute of Medical Biology, A*STAR, 3Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore

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Se presenta un protocolo para obtener matrices derivadas de células ricas en proteínas de la matriz extracelular, utilizando Crowders macromoleculares (MMC). Además, se presenta un protocolo que incorpora MMC en 3D generación de la piel de co-cultivo organotípicos, lo que reduce el tiempo de cultivo, manteniendo la madurez del constructo.

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Bioingeniería No. 114 queratinocitos fibroblastos la aglomeración macromolecular cultivo celular 2D 3D cultivo celular organotípicos piel co-cultivos matriz extracelular colágeno tipo I colágeno tipo IV colágeno tipo VII, unión dermoepidérmica
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