/
/
Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 00:46Stereotactic Engraftment of Glioma Cells into Mouse Striatum
- 02:52Mouse Transcardial Perfusion and Brain Harvesting
- 04:31Isolation of Glioma-infiltrating PBMCs
- 09:40Results: Flow Cytometric Gating Strategy Used to Identify Glioma-infiltrating Gr-1+/ CD11b+ Myeloid Cells and NK.1 + Cells
- 11:27Conclusion
Presented here is a straightforward method for the isolation and flow cytometric analysis of glioma-infiltrating peripheral blood mononuclear cells that yields time-dependent quantitative data on the number and activation status of immune cells entering the early brain tumor microenvironment.
