Journal
/
/
Assay הרגיש ביותר למדידה של Attachment Arenavirus התאים
JoVE 신문
면역학 및 감염병학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 면역학 및 감염병학
Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment
DOI:

08:34 min

March 02, 2016

,

Chapters

  • 00:05Title
  • 00:27Prepare Medium, Seed Cells in 48-well Plates, and Carry Out Virus-cell Attachment
  • 02:52Viral RNA Extraction
  • 04:22qRT-PCR Measurement of Viral Genome
  • 06:38Results: Arenavirus Cell Attachment Measurements
  • 08:08Conclusion

Summary

자동 번역

The first step in the arenavirus life cycle is attachment of viral particles to host cells. We report a quantitative (q)RT-PCR-based assay for ultrasensitive detection and quantitation of arenavirus attachment events.

Related Videos

Read Article