Journal
/
발생학
/
Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos
JoVE 신문
발생학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 발생학
Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos

DOI:
10.3791/53696-v

•

08:43 min

•

June 06, 2016

•

, , , , ,
1Department of Nanobiomedical Sciences and BK21 PLUS NBM Global Research Center for Regenerative Medicine,Dankook University, 2Insititute of Tissue Regeneration Engineering,Dankook University, 3Department of Biological Sciences,Ajou University, 4Department of Pharmacy,Sahmyook University, 5Department of Animal Biotechnology,Sahmyook University

Chapters

  • 00:05Title
  • 01:13Oviduct Isolation
  • 02:17Retrieval and Culturing of 2-Pronuclear (2-PN) Embryos
  • 03:46Microinjecting 2-PN Embryos
  • 06:29Results: Neogenin Expression Manipulations
  • 07:59Conclusion

Summary

자동 번역

자동 번역

The goal of this protocol is to describe a loss- and gain-of function method that is applicable to identify neogenin as a stage-specific receptor that leads to trophectoderm and inner cell mass differentiation in preimplantation mouse embryos.

Tags

Loss-of-functionGain-of-functionCell FatePreimplantationMouse EmbryosInner Cell MassDifferentiationStage-specificEmbryonic Stem CellReceptorLigand발생학Stem Cell BiologyAnimal ScienceReproductive BiotechnologyAnimal Cloning2-PN EmbryosCumulus CellsHyaluronidaseDenuding

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/53696?article_type=v

Methods for Precisely Localized Transfer of Cells or DNA into Early Postimplantation Mouse Embryos
check_url/kr/53696?article_type=v

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos
check_url/kr/53696?article_type=v

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
check_url/kr/53696?article_type=v

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos
check_url/kr/53696?article_type=v

A Novel Use of Three-dimensional High-frequency Ultrasonography for Early Pregnancy Characterization in the Mouse
check_url/kr/53696?article_type=v

Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos
check_url/kr/53696?article_type=v

A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation
check_url/kr/53696?article_type=v

Visualization of Cellular Electrical Activity in Zebrafish Early Embryos and Tumors
check_url/kr/53696?article_type=v

Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence
check_url/kr/53696?article_type=v

Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos

Read Article