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Chapters
- 00:05Title
- 00:29Fly Preparation
- 01:25Chamber Construction, Assembly, & Assay Setup
- 03:07Results: Female Flies’ Egg-Laying Preferences
- 03:29Conclusion
This protocol describes a high throughput assay for testing egg-laying preferences of Drosophila melanogaster at single-animal resolution. This assay provides a simple, efficient, and scalable platform to identify genes and circuit components that control a simple decision-making process.
Tags
High Throughput AssayEgg-laying PreferencesDrosophila MelanogasterGenesNeuronsChoice BehaviorInfectioningEggening ChoiceNarrow Food VialFly MediaFemale ProgeniesYeast PastePropionic AcidSpatulaSurface Of The Food MediumWet SurfaceLarvae BurrowingEgg-laying ExperimentsAcrylic Egg-laying ChambersPlastic SheetsBottom SurfaceAnesthetize FliesCarbon Dioxide PadRecover From Carbon DioxideAcclimate To Environment
