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1Cardiovascular and Aging Research, Department of Medicine, Division of Physiology, Faculty of Science,University of Fribourg, 2Kidney Control of Homeostasis,Swiss National Centre of Competence in Research, 3System Physiology, Department of Medicine, Division of Physiology, Faculty of Science,University of Fribourg
Chapters
- 00:05Title
- 00:52Preparing the Organ Bath System
- 02:01Isolation of Mouse Aortas
- 03:03DHE and DAF-2DA Staining
- 04:47En Face Mounting and Confocal Microscopic Imaging
- 06:42Results: eNOS-uncoupling in Obese Mice
- 07:56Conclusion
I denne artikel beskriver vi en tilpasset relativt let metode under anvendelse af fluorescens farvestof diaminofluorescein-2 diacetat (DAF-2DA) og dihydroethidium (DHE) for en face samtidig detektion og visualisering af intracellulær nitrogenoxid (NO) og superoxid anion (O 2 .- ) henholdsvis i frisk isoleret intakte aorta hos en fedme musemodel.
