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القشرية أكتين تدفق في خلايا T كميا بواسطة المكانية والزمانية صورة الارتباط الطيفي للهيكلة الإضاءة المجهر البيانات
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data
DOI:

09:09 min

December 17, 2015

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Chapters

  • 00:05Title
  • 00:50Microscope Set-up
  • 03:20Cell Imaging
  • 04:44Structured Illumination Microscopy (SIM) Image Reconstruction and Spatio-temporal Image Correlation Spectroscopy (STICS) Analysis
  • 07:12Results: Representative Structured Illumination Data and STICS Output
  • 08:36Conclusion

Summary

자동 번역

To investigate flow velocities and directionality of filamentous-actin at the T cell immunological synapse, live-cell super-resolution imaging is combined with total internal reflection fluorescence and quantified with spatio-temporal image correlation spectroscopy.

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