JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 00:49Epicardial Outgrowth Culture and Genetic Ablation
- 03:31Epicardial Labeling and Ex Vivo Migration Culture
- 05:17Results: Representative Epicardial Outgrowth Purity
- 06:23Representative Ex Vivo Migration Assay
- 06:56Conclusion
Here, we describe methods for isolating primary mouse epicardial cells by an outgrowth culture assay and assessing the functional migration of epicardial-derived cells (EPDC) using an ex vivo heart culture system. These protocols are suitable for identifying genetic and chemical modulators of epicardial epithelial-to-mesenchymal transition (EMT) and motility.
