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评估抗癌药物的特异性<em>体外</em

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Assessing Specificity of Anticancer Drugs In Vitro

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评估抗癌药物的特异性<em>体外</em

DOI:

10.3791/53752-v

•

09:39 min

•

March 23, 2016

•

Lan Kluwe^2,3

¹Department of Maxillofacial Surgery,University Medical Center Hamburg-Eppendorf, ²Department of Neurology,University Medical Center Hamburg-Eppendorf, ³Laboratory for Tumor Biology and Regenerative Medicine,University Medical Center Hamburg-Eppendorf

Chapters

00:05Title
01:00Mix Culture of Both Tumor Cells and Non-tumor Cells and Drug Treatment
02:44Preparing DNA for Digital PCR
04:51Digital PCR
06:22Calculating Tumor Cell Proportion
07:20Results: Dose-Dependent Change of Tumor Cell Proportion and Responses of Tumor/Non-Tumor Cells
08:14Conclusion

Summary

자동 번역

该协议的目的是评估使用同时含有肿瘤和非肿瘤细胞混合培养抗癌药物在体外的特异性。

Tags

Keywords: In Vitro Anticancer Drugs Tumor Cells Non-tumor Cells Specificity Drug Discovery Personalized Cancer Care Cell Culture Nilotinib Dose-dependent HotSHOT Method Cell Lysis PCR DNA

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