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analysera<em> In Vivo</em> Cell Migration använder Cell Transplantationer och Time-lapse avbildning i Zebrafish embryon

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Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos

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analysera<em> In Vivo</em> Cell Migration använder Cell Transplantationer och Time-lapse avbildning i Zebrafish embryon

DOI:

10.3791/53792-v

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11:39 min

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April 29, 2016

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Florence A. Giger, Julien G. Dumortier, Nicolas B. David

¹CNRS UMR8197 – INSERM U1024,IBENS, Institut de Biologie de l’École Normale Supérieure, ²Department of Physiology Development and Neuroscience,University of Cambridge

Chapters

00:05Title
00:45Preparation of Needles and Dishes for Cell Transplantation
02:13Injection of Donor Embryos
03:35Cell Transplantation
06:03Single Cell Transplantation
07:19Embryo Mounting
08:51Results: In Vivo Cell Migration in Zebrafish Embryos
10:45Conclusion

Summary

자동 번역

Combining cell transplantation, cytoskeletal labeling and loss/gain of function approaches, this protocol describes how the migrating zebrafish prospective prechordal plate can be used to analyze the function of a candidate gene in in vivo cell migration.

Tags

Cell Migration Cell Transplantation Time-lapse Imaging Zebrafish Embryos Cell Adenomyosis Cell Injection Micromanipulator Injection Needle Agarose Gel Transgenic Embryos Actin-binding Domain Mcherry Cell Migration Field

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