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Chapters
- 00:05Title
- 00:37Radiation-attentuated Sporozoite Isolation from Mosquito Salivary Glands
- 02:07Sporozoite Injection
- 03:13Skin and Draining Lymphs Node Dissection
- 04:49Cell Isolation
- 07:18Results: Intradermal RAS Injection Increases Myeloid Cell Migration to Mouse Ear Skin and dLN
- 08:01Conclusion
We describe here a protocol for isolating myeloid cells from mouse skin and draining lymph node following intradermal injection of Plasmodium sporozoites. Flow cytometry of collected cells provides a reliable assay to characterize the skin and draining lymph node inflammatory response to the parasite.
