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Chapters
- 00:05Title
- 00:5924-well Plate Preparation for High and Low Density Neuron Culture
- 01:48Brain Removal and Hippocampi Dissection of E16.5 – E17.5 Mouse Embryos
- 04:02Enzymatic Digestion and Separation into Single Neurons
- 06:53Plating of Neurons and Long Term Co-Culture
- 08:51Results: Experimental Manipulations of Low Density Neurons Grown in Co-Culture
- 10:23Conclusion
Low density cultures of primary hippocampal neurons usually require glia feeder layer to supply neurotrophic factors and sustain longevity. We describe here a simplified method to culture ultra-low density neurons on glass coverslips in the presence of a high density neuronal feeder layer, which facilitates investigation of specific neuronal-autonomous mechanisms.
