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Mikroskopi af fission Gær Seksuel Lifecycle
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Microscopy of Fission Yeast Sexual Lifecycle

Mikroskopi af fission Gær Seksuel Lifecycle

DOI:
10.3791/53801-v

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07:47 min

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March 09, 2016

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, , , ,
1Department of Fundamental Microbiology,University of Lausanne

Chapters

  • 00:05Title
  • 01:00Culturing Fission Yeast Strains for Mating Experiments
  • 03:31Preparing Agarose Pads and Mounting Cells for Imaging
  • 05:52Long-term Monitoring of the Reproductive Process in Fission Yeast
  • 07:20Conclusion

Summary

자동 번역

자동 번역

We provide a reproducible basic method for the long-term microscopy of the fission yeast sexual lifecycle. With minor adjustments described, the presented protocol allows research focus on different steps of the reproductive process.

Tags

Keywords: Fission YeastSexual LifecycleTime-lapse ImagingMatingPolarized GrowthCell-cell FusionMeiosisSporulationHomothallicHeterothallicMating Type SwitchingMinimum Sporulation MediumOptical DensityCell Culture

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