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Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis
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Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis

DOI:
10.3791/53822-v

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08:34 min

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June 03, 2016

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1Department of Medicine 3, Rheumatology and Immunology,Universitätsklinikum Erlangen, 2Nikolaus Fiebiger Center of Molecular Medicine,Universitätsklinikum Erlangen

Chapters

  • 00:05Title
  • 00:46Adult Male Mouse In Vivo Adipocyte Homeostasis Analysis
  • 04:58In Vitro Hypoxia-Influenced Adipocyte Homeostasis Analysis
  • 06:23Results: Representative Adipocyte Analyses
  • 07:59Conclusion

Summary

자동 번역

자동 번역

Adipose tissue (AT) can influence whole body homeostasis, therefore understanding the molecular mechanisms of adipocyte differentiation and function is of importance. We provide a protocol for gaining new insights into these processes by analyzing adipocyte homeostasis, differentiation and hypoxia exposure as a model for induced adipocyte apoptosis.

Tags

Keywords: AdipocyteAdipocyte HomeostasisAdipocyte DifferentiationAdipocyte ApoptosisHypoxiaObesityType II DiabetesPimonidazole HydrochloridePerigonadal Fat PadsHistological AnalysisImmunohistochemical AnalysisHematoxylinEosinProteinase KHydrogen PeroxideAntibodyBiotinylated Secondary Antibody

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