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Chapters
- 00:05Title
- 00:46B16.Flt.3L Melanoma Harvest
- 01:40Preparation of Splenic Single Cell Suspension from Tumor-bearing Mice
- 02:54Plasmacytoid Dendritic Cell (DC) Isolation
- 04:35CD8alpha+ and CD8alpha- DC Isolation
- 07:02Results: Representative Splenic DC Subset Analysis from B16.Flt3L Tumor-bearing Mice
- 08:10Conclusion
Distinct dendritic cell subsets exist as rare populations in lymphoid organs, and therefore are challenging to isolate in sufficient numbers and purity for immunological experiments. Here we describe a high efficiency, high yield method for isolation of all of the currently known major subsets of mouse splenic dendritic cells.
