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Chapters
- 00:05Title
- 01:13Preparation of Single Suspensions from Rat Liver
- 02:54Preparation and Enzyme Treatment of Comet Slides
- 04:44Unwinding Electrophoresis
- 06:37DNA Staining, Comet Visualization and Analysis
- 07:59Results: Measuring DNA Strand Breaks and Oxidative DNA Damage Using the Alkaline Comet Assay
- 09:27Conclusion
The alkaline comet assay measures DNA strand breaks in eukaryotic cells. By adding an Endonuclease III or human 8-oxoguanine-DNA-N-glycosylase digestion step, the assay can efficiently detect oxidative DNA damage. We describe methods for using these assays to detect DNA damage in rat liver.
