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A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors
 

A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors

Article DOI: 10.3791/53867-v 09:04 min March 15th, 2016
March 15th, 2016

챕터

요약

We describe a simple cell-based bioassay for detecting, quantifying and monitoring the activity of members of the vascular endothelial growth factor family of ligands. The assay uses chimeric receptors expressed in a factor-dependent cell line to provide a semi-quantitative or quantitative assessment of receptor binding and cross-linking by the ligand.

Tags

Keywords: Vascular Endothelial Growth Factor VEGF Bioassay Cell-based Assay VEGFR IL3 WEHI-3D BAF3 VegFR2-EPoR-BAF3 Cell Culture Cell Washing Cell Preparation
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