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Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues
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Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues
DOI:

13:03 min

June 03, 2016

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Chapters

  • 00:05Title
  • 01:10DNA-protein Cross-linking
  • 02:05Cell Lysis and Chromatin Fragmentation
  • 04:31Chromatin Immunoprecipitation
  • 05:49DNA Elution, Cross-link Reversal and Proteinase K Digestion
  • 07:33DNA Isolation Using DNA-binding Beads
  • 10:05Results: ChIP Protocols Investigate the Epigenetic Regulation of Cardiac Gene Transcription
  • 11:47Conclusion

Summary

자동 번역

A fine tuning regulation of gene transcription underlies embryonic cell fate decision. Herein, we describe chromatin immunoprecipitation assays used to investigate epigenetic regulation of both cardiac differentiation of stem cells and cardiac development of mouse embryos.

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