Individuazione di condizioni critiche per Immunostaining nel pisello afide embrioni: l'aumento del tessuto Permeabilità e diminuire la colorazione di fondo
07:00Results: Aphid Ovary Dissection and Whole-mount Immunostaining with Aphid-specific Conditions to Increase Tissue Permeability and Decrease Background Staining
A protocol of whole-mount immunostaining on aphid embryos is presented, in which critical conditions for decreasing background staining and increasing tissue permeability are addressed. These conditions are specifically developed for effective detection of protein expression in the embryonic tissues of aphids.