Journal
/
/
Séparation immunomagnétique de Sca1 Fat Depot spécifique<sup> haute</sup> Cellules souches adipeuses (CSA)
JoVE 신문
발생학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 발생학
Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells (ASCs)
DOI:

08:52 min

August 11, 2016

,

Chapters

  • 00:05Title
  • 00:45Subcutaneous (SQ) and Visceral (VIS) Fat Pad Isolation
  • 02:35Fat Pad Collagenase Digestion
  • 04:20Magnetic Cell Separation
  • 06:31Results: Fat Depot-dependent Expression of Collagenolytic Matrix Metalloproteinases (MMPs)
  • 07:52Conclusion

Summary

자동 번역

We present the techniques required to isolate the stromal vascular fraction (SVF) from mouse inguinal (subcutaneous) and perigonadal (visceral) adipose tissue depots to assess their gene expression and collagenolytic activity. This method includes the enrichment of Sca1high adipose-derived stem cells (ASCs) using immunomagnetic cell separation.

Related Videos

Read Article