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1Department of Biopathology and Medical Biotechnology, Section of Biology and Genetics,University of Palermo, 2Phase I-Early Clinical Trials Unit, Oncology Department,Antwerp University Hospital (UZA), 3Center for Oncological Research (CORE),Antwerp University, 4Department of Surgical, Oncological and Oral Sciences, Section of Medical Oncology,University of Palermo, 5Flemish Institute for Technological Research (VITO), 6CORE, Campus Groenenborger,Antwerp University, 7Molecular Pathology, Pathology Department,Antwerp University Hospital (UZA), 8Oncology Department,Antwerp University Hospital (UZA)
Chapters
- 00:05Title
- 00:49Exosome Isolation from Plasma Samples
- 03:11Reverse Transcription of Exosomal miRNAs
- 04:25RT-PCR Analysis of Exosomal miRNAs
- 06:43Results: miRNA Analysis from NSCLC Patient Plasma
- 07:43Conclusion
This protocol describes the feasibility to perform miRNA profiling in exosomes, released in plasma of NSCLC patients, through a commercial exosome isolation kit with Proteinase K and RNAse treatments, in order to avoid circulating miRNAs contamination and evaluate their biomarker features in NSCLC.
