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Chapters
- 00:05Title
- 01:07Preparation of Glycerol Gradients
- 02:42Ultracentrifugation of IAV
- 04:18SDS-PAGE of Pellet Fractions and Coomassie Staining
- 05:29Results: IAV In Vitro Core Disassembly Assay
- 06:45Conclusion
Disassembly of influenza A virus cores during virus entry into host cells is a multistep process. We describe an in vitro method to analyze the early stages of viral uncoating. In this approach, velocity gradient centrifugation is used to biochemically dissect the steps that initiate uncoating under defined conditions.
