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Chapters
- 00:05Title
- 00:51Tissue Embedding, Thin Sectioning and Mounting for LAM
- 04:14LAM
- 07:32Results: Identification of Genes Expressed Only in the Apomictic Egg Cell
- 08:33Conclusion
Here we present a protocol for laser-assisted microdissection of specific plant cell types for transcriptional profiling. While the protocol is suitable for different species and cell types, the focus is on highly inaccessible cells of the female germline important for sexual and apomictic reproduction in the crucifer genus Boechera.
