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Chapters
- 00:05Title
- 00:33Murine Embryonic Stem Cell (mESC) Culture and Passaging
- 03:16Embryoid Body (EB) Preparation and Grem2 Treatment
- 06:12Results: Grem2-induction of Cardiomyocyte Differentiation
- 08:00Conclusion
Generating cardiomyocytes from pluripotent stem cells in vitro allows access to large amounts of cardiac tissue in vitro for basic science and clinical applications. This protocol uses the atrializing factor Grem2 to both increase the numbers of cardiomyocytes obtained and to generate cardiomyocytes with an atrial phenotype.
