/

/

从背小鼠皮肤分离毛囊干细胞和表皮角质形成细胞

JoVE 신문
발생학

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE 신문 발생학

Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin

Please note that all translations are automatically generated. Click here for the English version.

从背小鼠皮肤分离毛囊干细胞和表皮角质形成细胞

DOI:

10.3791/53931-v

•

06:51 min

•

April 29, 2016

•

Despina Soteriou, Lana Kostic, Egor Sedov, Yahav Yosefzon, Hermann Steller, Yaron Fuchs

¹Department of Biology and Lokey Center, Laboratory of Stem Cell Biology and Regenerative Medicine,Technion Israel Institute of Technology, ²Strang Laboratory of Apoptosis and Cancer Biology,Howard Hughes Medical Institute, The Rockefeller University

Chapters

00:05Title
00:44Hair Follicle Isolation from Adult Epidermis
02:30Hair Follicle Cell Suspension Preparation
05:22Results: Representative Hair Follicle Cell Profiling
06:22Conclusion

Summary

자동 번역

An ideal model for studying adult stem cell biology is the mouse hair follicle. Here we present a protocol for isolating different populations of hair follicles stem cells and epidermal keratinocytes, employing enzymatic digestion of mouse dorsal skin followed by FACS analysis.

Tags

Hair Follicle Stem Cells Epidermal Keratinocytes Dorsal Mouse Skin Cell Isolation Cell Population Hair Follicle Cycle Skin Dissection Trypsin Digestion Hair Follicle Removal

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

转染，选择和人类诱导多能干细胞集落采摘TALEN靶向用GFP基因进入AAVS1安全港

集成的无人类诱导多能干细胞的生成方法头发衍生角质形成细胞

后生转换为安全和简单的方法来获得成人皮肤成纤维细胞胰岛素分泌细胞

从小鼠羊水细胞多能干细胞使用转座子系统的生产

水平整体安装：厚三维组织横截面皮肤的新型加工和成像方案

成人皮肤角质形成细胞的生成与培养

Pax7 和层粘连蛋白抗体免疫荧光法鉴定骨骼肌卫星细胞

秀丽线虫生殖的计算分析研究细胞核、蛋白质和细胞骨架的分布

一种简便有效的成人皮肤组织中人角质形成细胞的分离方法

从脐带血源性多能干细胞中提取三维皮肤有机细胞

Read Article