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Chapters
- 00:05Title
- 00:57Tissue Preparation
- 02:46IR-MALDESI Preparation/Calibration
- 04:09Deposition of Ice Matrix
- 05:29Mass Spectrometry Imaging Data Acquisition
- 09:07Results: Spatial Distribution of Lipids in Whole Mouse Preparations
- 09:52Conclusion
A mass spectrometry imaging (MSI) source operated at atmospheric pressure was developed by coupling mid-infrared laser desorption and electrospray post-ionization. Exogenous ice matrix was used as the energy-absorbing matrix to facilitate resonant desorption of tissue-related material. This manuscript provides a step-by-step protocol for performing IR-MALDESI MSI of whole-body neonatal mouse.
